Tzitzo, Tzouliana

2024-04-15T10:37:34Z

https://repository.ihu.edu.gr//xmlui/handle/11544/30340

3D

Bioinks

Organoids

Stem cells

Methodologies for 3D printing of Human neuronal cells

SID: 4402220011

2024-04-03

free

School of Economics, Business Administration & Legal Studies, MSc in Bioeconomy: Biotechnology and Law

The rapid evolution of 3D printing technology stands at the forefront of innovation in tissue engineering and regenerative medicine. This review outlines a comprehensive investigation into methodologies for the 3D printing of human neuronal cells, a pursuit that synergizes with the transformative capabilities of bioprinting technology. Bioprinting, and its potential to fabricate scaffolds, cells, tissues, and organs with reproducibility and high accuracy, serves as a pivotal element in advancing the frontiers of neuroregenerative therapies. Bioprinting technologies offer unique advantages and face distinct limitations. This review integrates fundamental principles and key aspects of frequently used bioprinting technologies, emphasizing recent advances in three-dimensional printing applications. As we delve into the intricacies of biomaterial selection, bioink formulation, and cutting-edge printing techniques within the context of neuronal cells, the review underscores the critical role of biocompatibility, cell viability, and the cellular microenvironment in influencing the printed product. The overarching objective of this research is to optimize the 3D printing process for human neuronal cells, aiming to enhance their viability, functionality, and organizational precision. By exploring diverse approaches, this study aspires to unlock the full potential of 3D printing, facilitating the creation of intricate neuronal architectures. The integration of 3D-printed neuronal cells into these diverse applications holds immense promise for revolutionizing the treatment landscape of neurodegenerative disorders. Through this comprehensive exploration of methodologies, recent advances, challenges, and future directions, this review aims to contribute to the scientific understanding of 3D-printed neuronal constructs, setting the stage for transformative advancements in the field of neuroregenerative medicine.

Petrakis, Spyros

IHU